Caiyun Yang, Yi Li, Qian Guo,Qiliang Lai,Tianling Zheng, Yun Tian.International Journal of Systematic and Evolutionary Microbiology, 2013. 63: 1621-1626.
A Gram-negative, short-rod-shaped and non-motile bacterium, strain 12C11T, was isolated from an oil-degrading consortium, enriched from the Fugong mangrove sediment, Fujian Province of China. Optimum growth was observed at 25 °C, at pH 7.5 and with 4% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis demonstrated that it shared highest similarity with members of the genusAlgoriphagus(97.5–93.4%), exhibiting 97.5% sequence similarity toAlgoriphagus ornithinivoransIMSNU 14014T, followed byAlgoriphagus vanfongensisKMM 6241T (97.2%); it shared <96 .0% sequence similarity with other members of the genus. levels of nucleotide sequence similarity betweengyrB(DNA gyrase subunit B) genes of strain 12C11TandA. vanfongensisKMM 6241T,A. ornithinivoransIMSNU 14014T, AlgoriphagusmarincolaSW-2T and Algoriphagushitonicola7-UAHT were 78.8, 78.6, 75.6 and 77.4%, respectively. Phylogenetic trees based on these housekeeping genes showed that strain 12C11Tand other Algoriphagus strains formed a distinct lineage. The dominant fatty acids were iso-C15:0(32.1%), C16:1ω7c/C16:1ω6c(11.6%), iso-C17:1I/anteiso-C17:1B (10.1%), iso-C17:03-OH (9.2%) and iso-C17:1ω9c/C16:0 10-methyl (7.1%), which accounted for 70.0% of the total fatty acids. DNA–DNA hybridization showed that strain 12C11Tshared low DNA–DNA relatedness withA. vanfongensisKMM 6241T andA. ornithinivoransIMSNU 14014T (30.7±0.9 and 30.5±1.8%, respectively). The G+C content of the chromosomal DNA of strain 12C11Twas 38.4 mol%. The major respiratory quinones were MK-7 (96.0%) and MK-6 (4.0%). According to its morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, the novel strain most appropriately belongs to the genus Algoriphagus, but can readily be distinguished from known Algoriphagus species. The nameAlgoriphaguszhangzhouensissp. nov. is proposed (type strain 12C11T=CGMCC 1.11027T=MCCC 1F01099T=DSM 25035T).
