作 者：Chen, J., Wang, W.H., Wu,F.H., He, E.M., Liu, X., Shangguan, Z.P., andZheng, H.L.

影响因子：5.295

刊物名称：Scientific Reports

出版年份：2015

卷：5 期：19

Hydrogen sulfide (H₂S) and nitric oxide (NO) are emerging as messenger molecules involved in themodulation of plant physiological processes. Here, we investigated a signalling network involving H₂Sand NO in salt tolerance pathway of barley. NaHS, a donor of H₂S, at a low concentration of either 50or 100μM, had significant rescue effects on the 150 mM NaCl-induced inhibition of plant growth andmodulated the K⁺/Na⁺balance by decreasing the net K⁺efflux and increasing the gene expressionof an inward-rectifying potassium channel (HvAKT₁) and a high-affinity K⁺uptake system (HvHAK₄).H₂S and NO maintained the lower Na⁺content in the cytoplast by increasing the amount of PM H⁺-ATPase, the transcriptional levels of PM H⁺-ATPase (HvHA₁) and Na⁺/H⁺antiporter (HvSOS₁). H₂Sand NO modulated Na⁺compartmentation into the vacuoles with up-regulation of the transcriptionallevels of vacuolar Na⁺/H⁺antiporter (HvVNHX₂) and H⁺-ATPase subunitβ(HvVHA-β) and increasedin the protein expression of vacuolar Na⁺/H⁺antiporter (NHE₁). H₂S mimicked the effect of sodiumnitroprusside (SNP) by increasing NO production, whereas the function was quenched with theaddition of NO scavenger. These results indicated that H₂S increased salt tolerance by maintainingion homeostasis, which were mediated by the NO signal.

Figure.11.A schematic model for H₂S enhances salt tolerance through NO-mediated maintenance of ion homeostasis inbarley seedling roots.