Jianqiang Su, Yanyan

Zhou, Qiliang Lai,Xinyi Li,Peiyan Dong,Xiaoru Yang,Bangzhou Zhang,Jinlong

Zhang,Xiaowei Zheng,Yun Tian, Tianling Zheng,International Journal of

Systematic and Evolutionary Microbiology,2013. 63: 2095-2100.

A taxonomic study was carried out on strain

SCSWE24^T, isolated from a seawater sample collected from the South

China Sea. Cells of strain SCSWE24^Twere Gram-negative, rod-shaped,

non-motile, moderately halophilic and capable of reducing nitrate to nitrite.

Growth was observed at salinities from 1.5 to 4.5% and at 4–37 °C; it was

unable to degrade gelatin. The dominant fatty acids (>15%) were summed

feature 3 (C_16:1ω7cand/or C_16:1ω6c; 50.4%)

and C_{16: 0}(21.1%). The G+C content of the chromosomal DNA was 58.8

mol%. 16S rRNA gene sequence comparisons showed that strain SCSWE24^Twas most closely related to an uncultured bacterium clone Tun3b.F5 (98%;

GenBank accession no.FJ169216), and showed 92% similarity to an endosymbiont bacterium from the

bone-eating wormOsedax mucofloris(clone Omu 9 c4791;

FN773233). Levels of similarity between strain SCSWE24^Tand type

strains of recognized species in the familyOceanospirillaceaewere less than 93%; the highest similarity was 92%, to bothAmphriteajaponicaJAMM 1866^Tand ‘Oceanicoccus sagamiensis’

PZ-5. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain

SCSWE24^Tformed a distinct evolutionary lineage within the familyOceanospirillaceae.

Strain SCSWE24^Twas distinguishable from members of phylogenetically

related genera by differences in several phenotypic properties. On the basis of

the phenotypic and phylogenetic data, strain SCSWE24^Trepresents a

novel species of a new genus, for which the nameSinobacteriumcaligoidesgen. nov., sp. nov. is proposed. The type

strain ofSinobacteriumcaligoidesis

SCSWE24T (=CCTCC AB 209289^T=LMG 25705^T=MCCC 1F01088^T).

An emended description ofAmphriteajaponicais also provided.

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