Caiyun

Yang, Yi Li, Qian Guo,Qiliang Lai,Tianling Zheng, Yun Tian.International

Journal of Systematic and Evolutionary Microbiology, 2013. 63:

1621-1626.

A Gram-negative, short-rod-shaped

and non-motile bacterium, strain 12C11^T, was isolated from an

oil-degrading consortium, enriched from the Fugong mangrove sediment, Fujian

Province of China. Optimum growth was observed at 25 °C, at pH 7.5 and with 4%

(w/v) NaCl. Comparative 16S rRNA gene sequence analysis demonstrated that it

shared highest similarity with members of the genusAlgoriphagus(97.5–93.4%), exhibiting 97.5% sequence similarity toAlgoriphagus ornithinivoransIMSNU 14014T, followed byAlgoriphagus vanfongensisKMM 6241T (97.2%); it shared <96 .0%

sequence similarity with other members of the genus. Levels of nucleotide sequence

similarity betweengyrB(DNA gyrase subunit B) genes of strain 12C11^TandA. vanfongensisKMM 6241T,A. ornithinivoransIMSNU 14014T,

AlgoriphagusmarincolaSW-2T and Algoriphagushitonicola7-UAHT

were 78.8, 78.6, 75.6 and 77.4%, respectively. Phylogenetic trees based on

these housekeeping genes showed that strain 12C11^Tand other

Algoriphagus strains formed a distinct lineage. The dominant fatty acids were

iso-C_15:0(32.1%), C_16:1ω7c/C_16:1ω6c(11.6%), iso-C_17:1I/anteiso-C_17:1B (10.1%), iso-C_17:03-OH (9.2%) and iso-C_17:1ω9c/C16:0 10-methyl (7.1%), which

accounted for 70.0% of the total fatty acids. DNA–DNA hybridization showed that

strain 12C11^Tshared low DNA–DNA relatedness withA. vanfongensisKMM 6241T andA. ornithinivoransIMSNU 14014T (30.7±0.9 and 30.5±1.8%,

respectively). The G+C content of the chromosomal DNA of strain 12C11^Twas 38.4 mol%. The major respiratory quinones were MK-7 (96.0%) and MK-6

(4.0%). According to its morphology, physiology, fatty acid composition and 16S

rRNA gene sequence data, the novel strain most appropriately belongs to the

genus Algoriphagus, but can readily be distinguished from known Algoriphagus

species. The nameAlgoriphaguszhangzhouensissp. nov. is proposed (type strain 12C11^T=CGMCC 1.11027^T=MCCC

1F01099^T=DSM 25035^T).

返回