Juan

Chen, Wen-Hua Wang, Ting-Wu Liu, Fei-Hua Wu, Hai-Lei Zheng*.Plant

Physiology and Biochemistry,2013.64: 41–51.

To study whether differential responses occur

in photosynthesis and antioxidant system for seedlings ofLiquidambar formosana,

an acid rain (AR)-sensitive tree species and Schima superba, an AR-tolerant

treespecies treated with three types of pH 3.0 simulated AR (SiAR) including

sulfuric-rich (S-SiAR), nitric-rich(N-SiAR), sulfate and nitrate mixed

(SN-SiAR), we investigated the changes of leaf necrosis, chlorophyllcontent,

soluble protein and proline content, photosynthesis and chlorophyll

fluorescence characteristics,reactive oxygen species production, membrane lipid

peroxidation, small molecular antioxidant content,antioxidant enzyme activities

and related protein expressions. Our results showed that SiAR

significantlycaused leaf necrosis, inhibited photosynthesis, induced superoxide

radical and hydrogen peroxide generation,aggravated membrane lipid

peroxidation, changed antioxidant enzyme activities, modifiedrelated protein

expressions such as Cu/Zn superoxide dismutase (SOD), L-ascorbate peroxidase

(APX, EC1. 11. 1. 11), glutathione S transferase (GST, EC 2. 5. 1. 18) and

Rubisco large subunit (RuBISCO LSU), alterednon-protein thiols (NPT) and

glutathione (GSH) content in leaves of L. formosana and S. superba.

Takentogether, we concluded that the damages caused by SiAR in L. formosana

were more severe and sufferedfrom more negative impacts than in S. superba.

S-SiAR induced more serious damages for the plants thandid SN-SiAR and N-SiAR.

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