作 者：Dai, Y.F.,Fang, W.Z., Lin, Q.X., Zhou, X.P.,Chen, X.L. 影响因子：0.918
刊物名称：Conservation Genetics Resources
出版年份：2015
卷：7 页码：635-638
Noninvasive DNA may be used in individual identification for counting or tracking animals. Here a multiplex PCR assay, in which 16 highly polymorphic microsatellite markers were chosen and allocated into four multiplex PCR panels, was described for the individual identification of the Chinese Egret (Egretta eulophotes)using molted feathers. In this study, the combined exclusion probability for unrelated individuals (PID) and for individuals with sibling
relationship (PID(sib)) were 7.02×10-19and 2.9×10-7, respectively. Molted feathers (n =59) ofE. eulophotesamplified by more than 7 loci were unambiguously identified as 43 different individuals (32 females and 11 males). The results indicated that our newly developed assay could be used for noninvasive individual identification in the vulnerable Chinese Egret and other Ardeids.
Fig.1.The combined exclusion probability for unrelated individuals (PID) and for individuals with sibling relationship (PID(sib)) based on16 microsatellite loci
