Mei-Hua Yang, Chih-Min Chen, Yong-Hua Hu, Cheng-Yi Zheng, Zhi-Cong Li, Lian-Lian Ni, Li Sun, Qing-Xi Chen.Journal of Bioscience and Bioengineering, 2013. 115: 514-517.
4-Dimethylaminobenzaldehyde-thiosemicarbazone (DABT) and 4-dimethylaminobenzaldehyde-N-phenyl-thiosemicarbazone (DABPT) were synthesized and established by1H and13C NMR and mass spectrum. Both compounds wereevaluated for their inhibition activities on mushroom tyrosinase and their anti-tyrosinase kinetics was investigated. Theresults showed that both compounds exhibited significant inhibitory effects on activity of monophenolase and diphenolase;DABT and DABPT decreased the steady-state rate with 1.54 mM and 1.78 mM as their IC50 values respectively. Theinhibitory effects of diphenolase activity exhibited sharp in a dose-dependent manner and their IC50 values were estimatedas 2.01 mM and 0.80 mM, respectively. Kinetic analysis showed that their inhibition mechanism was reversible. Theinhibition type of DABT was mix-type with inhibition constants KI [ 1.77 mM and KIS [ 6.49 mM, while that of DABPTdisplays non-competitive with the inhibition constant KI [ 0.77 mM].
