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代表性论文

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Development and cross-species transferability of 23 microsatellite markers from the vulnerable Chinese Egret (Egretta eulophotes) using 454 sequencing

发布时间:2014-01-01

Yufei Dai, Xiaoping Zhou, Wenzhen Fang , Qingxian Lin, Xiaolin Chen.Conservation Genet Resour,2013. 5:1035–1038.

The Chinese Egret (Egretta eulophotes) is avulnerable water bird breeding on offshore islands. Here, 23new microsatellite loci were isolated using 454 pyrosequencing, and were tested in 32 individuals from a single population in Fujian province of China. The number of alleles per locus ranged from 2 to 9. Observed heterozygosityand expected heterozygosity ranged from 0.094 to0.844 and 0.091 to 0.840, respectively. All loci showed Hardy–Weinberg equilibrium with the exception of three loci (Ee10, Ee17 and Ee23), possibly because of nullalleles. The new loci also were confirmed a high cross amplification success rate in the other seven Ardeid species. These new microsatellite data will provide a major resource for future conservation genetics studies on this vulnerable egret and the other Ardeids.

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