Xiaowei Zheng, Bangzhou

Zhang, Jinlong Zhang, Liping Huang, Jing Lin, Xinyi Li, Yanyan Zhou, Hui Wang,

Xiaoru Yang, Jianqiang Su, Yun Tian, Tianling Zheng.Appl Microbiol Biotechnol,

2013. 97:9207– 9215

A

strain O4-6, which had pronounced algicidaleffects to the harmful algal bloom

causing alga Phaeocystisglobosa, was isolated from mangrove sediments in

theYunxiao Mangrove National Nature Reserve, Fujian,China. Based on the 16S

rRNA gene sequence and morphological characteristics, the isolate was found to

be phylogenetically related to the genus Streptomyces and identified

asStreptomyces malaysiensis O4-6. Heat stability, pH tolerance, molecular

weight range and aqueous solubility weretested to characterize the algicidal

compound secreted fromO4-6. Results showed that the algicidal activity of

thiscompound was not heat stable and not affected by pHchanges. Residue

extracted from the supernatant of O4-6fermentation broth by ethyl acetate, was

purified bySephadex LH-20 column and silica gel column chromatography before

further structure determination. Chemicalstructure of the responsible compound,

named NIG355,was illustrated based on quadrupole time-of-flight

massspectrometry (Q-TOF-MS) and nuclear magnetic resonance(NMR) spectra. And

this compound showed a strongeralgicidal activity compared with other reported

algicides.Furthermore, this article represents the first report of analgicide

against P. globosa, and the compound may bepotentially used as a bio-agent for

controlling harmful algalblooms.

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