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代表性论文

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Development and cross-species transferability of 23 microsatellite markers from the vulnerable Chinese Egret (Egretta eulophotes) using 454 sequencing

发布时间:2014-01-01

Yufei Dai, Xiaoping

Zhou, Wenzhen Fang , Qingxian Lin, Xiaolin Chen.Conservation Genet Resour,2013.

5:1035–1038.

The Chinese Egret (Egretta eulophotes) is avulnerable water

bird breeding on offshore islands. Here, 23new microsatellite loci were

isolated using 454 pyrosequencing, and were tested in 32 individuals from a

single population in Fujian province of China. The number of alleles per locus

ranged from 2 to 9. Observed heterozygosityand expected heterozygosity ranged

from 0.094 to0.844 and 0.091 to 0.840, respectively. All loci showed Hardy–Weinberg

equilibrium with the exception of three loci (Ee10, Ee17 and Ee23), possibly

because of nullalleles. The new loci also were confirmed a high cross amplification

success rate in the other seven Ardeid species. These new microsatellite data

will provide a major resource for future conservation genetics studies on this vulnerable

egret and the other Ardeids.

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