Zhang L.-D.; Song L.-Y.; Dai M.-J.; Liu J.-Y.; Li J.; Xu C.-Q.; Guo Z.-J.; Song S.-W.; Liu J.-W.; Zhu X.-Y. and Zheng H.-L. 2023. Journal of Hazardous Materials 459.

Mangrove Avicennia marina has the importantly potential for cadmium (Cd) pollution remediation in coastal wetlands. Unfortunately, the molecular mechanisms and transporter members for Cd uptake by the roots of A. marina are not well documented. In this study, photosynthetic and phenotypic analysis indicated that A. marina is particularly tolerant to Cd. The content and flux analysis indicated that Cd is mainly retained in the roots, with greater Cd influx in fine roots than that in coarse roots, and higher Cd influx in the root meristem zone as well. Using transcriptomic analysis, a total of 5238 differentially expressed genes were identified between the Cd treatment and control group. Moreover, we found that 54 genes were responsible for inorganic ion transport. Among these genes, AmHMA2, AmIRT1, and AmPCR2 were localized in the plasma membrane and AmZIP1 was localized in both plasma membrane and cytoplasm. All above gene encoding transporters showed significant Cd transport activities using function assay in yeast cells. In addition, the overexpression of AmZIP1 or AmPCR2 in Arabidopsis improved the Cd tolerance of transgenic plants. This is particularly significant as it provides insight into the molecular mechanism for Cd uptake by the roots of mangrove plants and a theoretical basis for coastal wetland phytoremediation.

Phylogenetic tree and heatmap of expressed abundance for HMA and ZIP family members in A. marina. Phylogenetic trees of HMAs (A) and ZIPs (C). The black dot represents the Arabidopsis gene, the green dot represents the rice gene, and the red dot represents the A. marina gene. The TPM values of AmHMAs (B) and AmZIPs (D) were obtained by transcriptome sequencing.